Use of Maldi-Tof MS biosensor in Brazilian kefir grains microbial assessment

Roberta Oliveira Viana, Karina Teixeira Magalhães-Guedes, Disney Ribeiro Dias, Rosane Freitas Schwan


The aim of this study was to identify the microorganisms associated with Brazilian kefir grains using the a new methodology to microorganisms rapid diagnosis, Maldi-Tof MS biosensor. A total of 357 microorganisms were isolated, and 31 were yeasts and 327 were bacteria (divided into lactic and acetic bacteria’s). The microbial population identified in Brazilian kefir grains was Lactobacillus paracasei, Saccharomyces cerevisiae, Lactobacillus plantarum, Acetobacter pasteurianus and Acetobacter syzygii. Rapid diagnosis of microorganisms is decisive to guarante adequate identification in biológical samples. Maldi-Tof biosensor is available to enable faster diagnosis for microbial identification. This study demonstrated a rapid and accurate identification of the Brazilian kefir grains microorganisms using this biosensor. In conclusion, the Maldi-Tof MS technology can facilitate the microbiological control in a fermentation process using kefir grains as starter cultures.


microorganisms; bacteria’s; yeast; biosensor


Cho Y-J, Kim D-H, Jeong D, Seo K-H, Jeong HS, Lee HG & Kim H (2018) Characterization of yeasts isolated from kefir as a probiotic and its synergic interaction with the wine byproduct grape seed flour/extract. LWT - Food Science and Technology, 90:535-539.

Corona O, Randazzo W, Miceli A, Guarcello R, Francesca N, Esten H, Moschetti G & Settanni L (2016) Characterization of kefir-like beverages produced from vegetable juices. LWT - Food Science and Technology, 66:572-581.

Gaudreau AM, Labrie J, Goetz C, Dufour S & Jacques M (2018) Evaluation of MALDI-TOF mass spectrometry for the identification of bacteria growing as biofilms. Journal of Microbiological Methods, 145:79-81.

Guzel-Seydim Z, Wyffels JT, Seydim AC & Greene AK. (2005) Turkish kefir and kefir grains: microbial enumeration and electron microscobic observation. International Journal of Dairy Technology. 58:25-29.

Irigoyen A, Arana I, Castiella M, Torre P & Ibáñez FC (2005) Microbiological, physicochemical, and sensory characteristics of kefir during storage. Food Chemistry, 90:613-620.

Lee MY, Ahn KS, Kwon OK, Kim MJ, Kim MK, Lee IY, Oh SR & Lee HK (2007) Anti-inflammatory and anti-allergic effects of kefir in a mouse asthma model. Immunobiology, 212:647-654.

Lima N, Rodrigues P, Soares C, Santos C, Paterson R & Venâncio A (2008) Modern Polyphasic Methods that Include Maldi-Tof Analyses for Fungal Identifications and Authentications. European Culture Collections' Organization Meeting XXVII (ECCO XXVII), 10th - 11th June, Ghent, Belgium. p.2.

Magalhães KT, Dias, DR, Pereira GVM, Oliveira JM, Domingues L, Teixeira JÁ, Silva JBA & Schwan RF (2011a) Chemical composition and sensory analysis of cheese whey-based beverages using kefir grains as starter culture. International Journal of Food Science and Technology, 46:871-878.

Magalhães, K. T.; Dragone, G.; Pereira, G. V. M.; Oliveira, J. M.; Domingues, L.; Teixeira, J. Á, Silva JBA & Schwan RF (2011b) Comparative study of the biochemical changes and volatile compounds during the production of novel whey-based kefir beverages and traditional milk kefir. Food Chemistry, 126:249-253.

Magalhães KT, Pereira GVM, Campos CR, Dragone G & Schwan RF (2011c) Brazilian kefir: structure microbial communities and chemical composition. Brazilian Journal of Microbiology, 42:693-702.

Magalhães KT, Pereira MA, Nicolau A, Dragone G, Domingues L, Teixeira JA Silva JBA & Schwan RF (2010a) Production of fermented cheese whey-based beverage using kefir grains as starter culture: Evaluation of morphological and microbial variations. Bioresource Technology, 101:8843-8850.

Magalhães KT, Pereira GVM & Schwan RF (2010b) Microbial communities and chemical changes during fermentation of sugary Brazilian kefir. World Journal of Microbiology and Biotechnology, 1:1-10.

Mayoral TN, Andrés AGA, Carretero SF, Fernández RC & Pajares MSJ (2018) Cervicofacial lymphadenitis due to Mycobacterium mantenii: rapid and reliable identification by MALDI-TOF MS. New Microbes and New Infections, 22:1-3.

Medrano M, Pérez PF & Abraham AG (2012) Kefiran antagonizes cytopathic effects of Bacillus cereus extracellular factors. International Journal of Food Microbiology, 122:1-7.

Pasternak J (2012) Novas metodologias de identificação de micro-organismos: Maldi-Tof. Einstein (São Paulo). 10:118-119.

Puerari C, Magalhães KT & Schwan RF (2012) New cocoa pulp-based kefir beverages: Microbiological, chemical composition and sensory analysis. Food Research International, 48:634-640.

Quintilla R, Kolecka A, Casaregola S, Daniel HM, Houbraken J, Kostrzewa M, Boekhout T & Groenewald M (2018) MALDI-TOF MS as a tool to identify foodborne yeasts and yeast-like fungi. International Journal of Food Microbiology, 266:109-118.

Rodrigues KL, Caputo LRG, Carvalho JCT, Evangelista J & Schneedorf JM (2005) Antimicrobial and healing activity of kefir and kefiran extract. International Journal of Antimicrobial Agents, 25:404-408.

Roos J & Vuyst L (2018). Acetic acid bacteria in fermented foods and beverages. Current Opinion in Biotechnology, 49:115-119.

Simova E, Beshkova D, Angelov A, Hristozova TS, Frengova G & Spasov Z (2002) Lactic acid bacteria and yeasts in kefir grains and kefir made from them. Journal Industrial of Microbiology and Biotechnology 28:1-6.

Vinderola CG, Duarte J, Thangavel D, Perdigón G, Farnworth E & Matar C (2005) Immunomodulating capacity of kefir. Journal of Dairy Research, 72:195-202.


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